Figure 1.

An experimental workflow. Contamination cleanup with Cod uracil-DNA N-glycosylase (Cod UNG) and preamplification can be performed separately or as one combined step. In this study, contamination cleanup was performed together with the preamplification step. Samples were UNG treated by adding Cod UNG to the preamplification master mix and by subsequently incubating the samples for 5 min at room temperature before initiating preamplification. The pre-denaturation step at 95 °C for 10 min that activate the polymerase also irreversibly inactivate Cod UNG. dUTP: deoxyuridine triphosphate; qPCR: quantitative real-time polymerase chain reaction.