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The cell apoptosis was detected by flow cytometry using Annexin V-FITC/PI dual staining. A549 cells were treated with 4B, 6i, 4D, and 6u at 2, 4, and 8 μM for 48 h, and then stained with Annexin V-FITC and PI for 15 min at 37 °C. The proportions of the living cells, the early apoptosis cells, and the late apoptotic cells were analyzed by FCS Express 5.0.
