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. 2018 Oct 13;19(10):3149. doi: 10.3390/ijms19103149

Figure 2.

Figure 2

Zerumbone (ZER) decreases expression of melanogenesis-related genes and proteins upon SCF stimulation in G361 human melanoma cells. (A) Protein expression levels of microphthalmia-associated transcription factor (MITF) and tyrosinase in presence and absence of ZER. Cells were pre-treated with 10 μM ZER for 1 h, and then incubated with 10 nM SCF for different time intervals as indicated; (B) mRNA expression levels of MITF in presence and absence of ZER upon SCF stimulation. Cells were pre-treated with ZER (10 μM) for 1 h, followed by incubation with 10 nM SCF for different time intervals as indicated. Values represent mean ± SD of two independent experiments performed in triplicate; * p < 0.05 and ** p < 0.01.; (C) Tyrosinase and TYRP1 mRNA levels in the absence or presence of ZER upon SCF stimulation. ZER (10 μM) was pre-treated for 1 h and then cells were further incubated with 10 nM of SCF for indicated time. Values represent mean ± SD of two independent experiments performed in triplicate; * p < 0.05 and ** p < 0.01.