Figure 3.

Effect of combination treatment with SOR and BA on cell cycle arrest in AsPC-1 cells. (A) Representative cell cycle analyzed by FACS of AsPC-1 cells after treatments with sorafenib (5 µM) and betulinic acid (6 µM) alone and in combination (n = 3). (B) Representative immunoblot of p21, c-Myc, cyclin D1, and cyclin B1 expression from AsPC-1 cells treated with sorafenib (5 µM) and betulinic acid (6 µM) alone and in combination (n = 3). Actin served as a loading control. Data are presented as means ± SD. * p < 0.05, ** p < 0.01 compared with the sorafenib treatment group and betulinic acid treatment group. All experiments were repeated at least three times.