Skip to main content
. 2018 Oct 17;19(10):3207. doi: 10.3390/ijms19103207

Figure 2.

Figure 2

Zeb2 overexpression induces expression of proteins characteristic of the myofibroblast phenotype in primary rat cardiac fibroblasts. P1 cells were transduced with Ad-EGFP or Ad-HA-Zeb2 and incubated for 96 h prior to Western blot analysis. α-Tubulin was used as a loading control. Rabbit polyclonal anti-Zeb2 antibody was used to determine Zeb2 expression levels (Panel A). Ectopic expression of Zeb2 increased the expression of (A) α-SMA, (B) SMEmb and (C) ED-A fibronectin. Right panels are histographic representations of respective expression of markers relative to α-tubulin. The data shown are from n = 4 independent experiments for α-SMA and SMemb, and n = 3 for ED-A fibronectin. * p < 0.05 vs. Ad-EGFP. Error bars represent SEM. Data were analyzed by performing Student’s t-tests.