Fig. 1.

In vitro propagation using nodal explants and ex vitro rooting of L. inermis. a Multiple shoot induction of nodal segment explant cultured on MS medium supplemented with 0.5 μM each of BA and 2-iP (bar = 0.6 cm), b Axillary bud proliferation on MS medium supplemented with BA 0.5 μM with 2-iP 0.5 μΜ (bar = 0.5 cm), c Ex vitro rooted plantlets in different length class (< 0.5–1.0, 1.1–2.0, 2.1–3.0, 3.1–4.0, 4.1–5.0) treated with 0.44 mM of NAA (bar = 0.3 cm), d ex vitro rooted plantlets produced by treating treated with 0.44 mM of NAA (bar = 0.3 cm), e ex vitro rooted microshoots after 4 weeks of potting (bar = 0.26 cm) and f lawsone extracted from mother plant (CEP 16), different in vitro stages and micropropagagted plants of varying ages (4-week old, 2-month old, 6 month old and 1 year-old)