Table 3.
Effect of 0.5 µM BA and different concentrations of (KIN, 2-iP) on shoot multiplication and elongation using nodal explants of L. inermis
| Cytokinin conc. (µM) | Explant response (%) | No. of shoots/explant | Shoot length (cm) |
|---|---|---|---|
| BA 0.5 µM (control) | 71.67 ± 2.89b | 18.33 ± 0.58 cd | 4.90 ± .361 cd |
| KIN | |||
| 0.25 | 20.00 ± 1.00f | 5.00 ± 2.43f | 1.85 ± 0.24f |
| 0.5 | 60.67 ± 3.06c | 12.00 ± 1.00d | 5.00 ± 0.40d |
| 1 | 59.00 ± 2.29c | 8.00 ± 1.73e | 3.37 ± 0.32e |
| 2.5 | 41.67 ± 6.66d | 8.67 ± 1.53e | 3.20 ± 0.92e |
| 5 | 23.00 ± 1.00e | 6.00 ± 2.65e | 1.87 ± 0.32f |
| 2-iP | |||
| 0.25 | 72.00 ± 2.45b | 25.45 ± 1.34c | 5.54 ± 1.20c |
| 0.5 | 89.67 ± 0.58a | 43.67 ± 1.53a | 12.57 ± 0.74a |
| 1 | 86.00 ± 1.00a | 39.00 ± 1.00b | 11.97 ± 0.85a |
| 2.5 | 75.67 ± 5.5b | 38.33 ± 2.52b | 10.53 ± 0.81b |
| 5 | 73.00 ± 2.65b | 26.67 ± 1.53c | 6.40 ± 1.25c |
| Treatment df (n − 1) = 9 | 50.15*** | 239.78*** | 92.42*** |
| Cytokinin type (T) df (n − 1) = 1 | 45.09** | 121.61** | 64.34** |
| Cytokinin conc.(C) df (n − 1) = 4 | 5.89* | 3.46NS | 5.28NS |
| TxC df (n − 1) = 8 | 5.33* | 12.43*** | 7.85** |
***Significant at p < 0.001 level; **Significant at p < 0.001 level; *Significant at p < 0.05 level, NS non significant; Means within column followed by same letter are not significantly (p < 0.05) different as determined by Duncan’s Multiple Range test (DMRT)