Table 4.
Summary of in vitro studies of L. inermis
| References | Explant and mode of regeneration | Treatment/medium | Result | Remarks |
|---|---|---|---|---|
| Bakkali et al. (1997) | Internode segments (1–2 cm length) from 6 to weeks old axenic seedlings | MS + 0.4 mg l−1 NAA + 2 mg l−1 BA + 0.25 µM of FeSO4 for shoots and hormone-free ½ MS solid medium for in vitro rooting | 3.8 shoots | Low frequency of multiplication. As the explant is seedling based quality of propagules cannot be ensured |
| Rout et al. (2001) | Apical shoot and node of actively growing young stems from green house growing plant | MS + 0.25 mg l−1 + BA, 0.25 mg l−1 + Kn + 0.5 mg l−1 ascorbic acid for shoot and 0.25 mg l−1 IBA with 2% (w/v) sucrose for in vitro rooting | 1.18 shoots | Low frequency of multiplication |
| Ram and Shekhawat (2011) | Apical shoot tip, below apical nodal segments and woody nodes and fresh hard nodal segments of mature plant (direct) | MS + 0.25 mg l−1 BA + 0.25 mg l−1 + Kn + 0.1 mg l−1 IAA + 50 mg l−1 ascorbic acid + 25 mg l−1 ammonium sulphate for shoot and 300 mg l−1 IBA and 100 mg l−1 NOA for rooting | 28.5 shoots with basal callus | High concentration of excess additives with cytokinins in combination with auxin were used for rising multiple shoots. Taken long (8–10) days for bud break even though medium was supplemented with hormones and addition of additives with higher concentration |
| Singh et al. (2012) | Apical shoot tip and meristems nodes of actively growing young stems from mature plant (indirect) | MS + 0.2 mg l−1 BAP + 0.2 mg l−1 Kn +0.2 mg l−1 CW for shooting and ½ MS + 200 mg l−1 AC + 0.5 mg l−1 NAA for rooting | 4.7 shoots | Low frequency of multiplication |
| Moharana et al. (2017) | Nodal segments belonging to different position from shoot apex from mature plant | MS + 1.0 mg l−1 BA for shoots and MS medium with free hormone for rooting | 7.8 shoots | Low frequency of multiplication |
| Present study | 4th and 5th nodes from elite plant (direct) | MS + 0.5 µM BA + 0.5 µM 2-iP for shoots and 0.44 mM NAA for rooting | 43.67 shoots | Used low concentration of cytokinin combination for multiplication, Continuous subculture attained high frequency (43.67) shoot multiplication. Use of auxin in multiplication medium, avoided-reduced callusing, chances for variation. Simple ex vitro rooting system was standardized. Evaluated the best size shoots for rooting. Time taken for rooting is less over previous method |