Fig. 1.

Characterization of isolated human dermal fibroblasts: Polystyrene microparticles coupled to the kappa light chain of mouse immunoglobulin (BD CompBead plus, The Scientific Group) were incubated with these primary as well as the fluorochrome-conjugated secondary antibodies as primary cells to serve as the positive staining control (A). Primary cells in its mid-logarithmic phase were incubated with mouse anti-human polyclonal antibodies for CD44, CD90, CD133 and CD166 followed by FITC-conjugated rabbit anti-mouse antibody for 30 min each on ice (B). Unstained BD CompBead plus and primary cells were the controls.