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. Author manuscript; available in PMC: 2018 Nov 2.
Published in final edited form as: J Drug Des Res. 2018 Aug 13;5(2):1070.

Figure 12.

Figure 12

Compound CX06 reduces the rate of capsid assembly and destabilizes the assembled capsid core in vitro. (A) Capsid assembly was monitored by an increase in turbidity using a spectrophotometer at 350 nm. Capsid was used at a final concentration of 30 μM, and compounds CX06 and PF74 at a final concentration of 50 μM. The presence of compound CX06 prevents the assembly of the capsid. (B) IN-GFP and CypA-DsRed double labeled HIV-1 viruses were bound to a coverglass, permeabilized with saponin (100ug/ml, 1 min) and washed. Viral Cores were imaged in the presence of a compound (5μM PF74 or 10μM CX06) or vehicle DMSO control at the indicated time. Average number of CypA-DsRedspots from four random fields was calculated at initial time point (0 min) as 100% value and calculated every minute for over 25 minutes. The CypA-DsRedspot numbers were divided by the number at initial time point and were presented as percent intact cores.