Figure 2.

Use of transwell membranes in advanced culture models. Monoculture for permeation experiments (A), indirect contact (B) and direct or indirect contact (C) co-culture of only one cell type in each chamber. Cells can be cultured or separated by matrices that may either be acellular (D) or contain one (E) or several types of cells (E, F). Co-culture systems may consist of two and more cell types in the apical compartment (G), co-culture of two and more cell types in the apical compartment in indirect culture with one cell type in the basolateral compartment (H), co-culture of one cell type in the apical and several types of cells in the basolateral compartment (I), combined direct contact and indirect contact culture (J), direct contact culture of several cell types in the apical compartment and one type in the basolateral compartment (K). The separation line in H, J and K indicates that different cell types in monoculture can be used in the basolateral compartment or in the apical compartment (E).