Figure 3.

Particle and biological parameter that were identified to play a role in in silico modelling of metal oxide NPs (according to the meta-analysis by Ha et al. [105]). Parameters can be influenced by the use of advanced cell culture models, either by medium composition (M) or by the culture method (C). Medium composition may have an influence on aggregation (hydrodynamic size) and influence the dose that reaches the cell. In addition, surface parameters may be changed. The culture method influences mainly cellular differences by increasing cell differentiation and the exposure time as physiologically relevant culture methods usually enable exposure for longer time periods.