Fig. 2.

STAT3 localizes to the ER and MAM compartments and interacts with IP3R3. a Whole-cell lysates from MDA-MB-468 cells were fractionated and analysed by Western blot. Representative of at least five independent experiments. b Co-immunoprecipitation of STAT3 and IP3R3. Whole-cell lysates or ER and MAM fractions were subjected to immunoprecipitation with anti-STAT3 antibodies (S3) or with control IgG, and blotted with the indicated antibodies. Representative of at least five independent experiments. WCL whole-cell lysate, ER endoplasmic reticulum, MAM mitochondrial-associated membranes, T total extract. c Whole-cell lysates from MDA-MB-468 cells were treated with the indicated amounts of Proteinase K (PK) in the presence or absence of 3% Triton-X 100 (Tx), and analysed by Western blot. Representative of five independent experiments