Fig 2. Analysis of cross talk of the two lasers In vitro.

Phantoms have been created containing 10¹, 5x10¹, 10², 5x10², 10³ and10⁴ nM of fluorophore Cy 5.5 (n = 6) and Cy 7.0 (n = 6). All phantoms were examined with the fiberscope tip being at 3mm (green dots and lines), 11mm (red dots and lines) and 50mm (blue dots and lines) The Cy 5.5 phantoms were examined with the 680 mode (Fig 2, 1) and 750 mode (Fig 2, 2), and the Cy 7.0 were examined with 680 mode (Fig 2, 3) and 750 mode (Fig 2, 4). The images of the phantoms were recorded and their image intensity were calculated with image J and recorded as intensity arbitrary units (AU). The horizontal line represents the electronic noise of the endoscopy system.