Fig. 6. The rADAMTS13-loaded murine platelets inhibit the rate of thrombus formation in mesenteric arterioles of Adamts13−/− mice after FeCl3 injury.
A. Schematic representation of mesenteric arteriole network and the position where a piece of filter paper with 10% FeCl3 (wt./vol.) was placed. B. The means and standard errors of the means (SEM) of the rates of thrombus formation as a function of time in each group were quantified off-line using the NIS Elements software and plotted using the Prism 7 software. C. Time lapses demonstrate the rates of thrombus formation induced by 10% FeCl3 injury to the mesenteric arterioles in Adamts13−/− mice after being transfused with 10% of rADAMTS13-loaded platelets (rA13-Plts), transgenic platelets (TG-Plts) expressing rADAMTS13, and Adamts13−/− platelets (KO-Plts) or in wild type mice (WT). All platelets (both infused and endogenous) were labeled with a rhodamine-6G. Representative digital images at 0, 5, 10, and 20 minutes in four different experimental groups are shown.