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The 1H-15N backbone CSPs obtained by comparison of the His–Fe–His and His–Fe–Lys forms of Fe(II) PPDME GlbN-A (pH 9.8) depicted on the His–Fe–His structure (PDB ID 4MAX). Blue (CSP < 0.95 ppm), gold (0.95 ppm < CSP < 1.40 ppm) and red (1.40 ppm < CSP). The average CSP is 0.50 ppm and the standard deviation is 0.45 (Figure S16). Backbone amides in grey could not be assigned at this alkaline pH.
