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(A) Dioxygen binding monitored by optical absorbance spectrophotometry. A ~5.4 μM ferric GlbN sample at pH 7.1 (FeIII, dashed trace) was incubated with a catalytic ferredoxin (Fd) reduction system. Gradual conversion of FeIII GlbN to oxy GlbN (FeII–O2) occurred in 10 min (solid traces). (B) Spectral overlay of FeII–O2 GlbN (solid trace) and FeII–O2 GlbN-A (dash-dot trace) prepared with the Fd reduction system.
