Homeostatic epidermal stem cell self-renewal is driven by local differentiation

. Author manuscript; available in PMC: 2019 Nov 1.

Published in final edited form as: Cell Stem Cell. 2018 Sep 27;23(5):677–686.e4. doi: 10.1016/j.stem.2018.09.005

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Dulbecco’s Phosphate Buffered Saline (PBS)	Thermo Fisher	Cat#14190144
Mitomycin C (MMC)	RPI	Cat#M92010
Demecolcine	Sigma-Aldrich	Cat#D7385
Dimethylsulfoxide (DMSO)	R&D	Cat#AB03091
Tamoxifen	Sigma-Aldrich	Cat#T5648
Experimental Models: Organisms/Strains
Mouse: K14-H2BPAmCherry	Rompolas et al., 2016	N/A
Mouse: K14-actinGFP	Vaezi et al., 2012	N/A
Mouse: K14-H2BCerulean	This paper	N/A
Mouse: mKO2-hCdt1(30/120)	Sakaue-Sawano, et al., 2008	N/A
Mouse: K14-H2BmCherry	Mesa et al., 2015	N/A
Mouse: B6.Cg-Ptprc^a Tg(UBC-PA-GFP) 1Mnz/J	The Jackson Laboratory	JAX: 022486
Mouse: Gt(ROSA)26Sor^{tm1 (D1A)Jpmb}/J	The Jackson Laboratory	JAX: 006331
Mouse: T g(KRT 14-cre/ERT)20Efu/J	The Jackson Laboratory	JAX: 005107
Mouse: B6.Cg-Tg(HIST1 H2B/EGFP)1 Pa/J	The Jackson Laboratory	JAX: 006069
Software and Algorithms
Fiji (ImageJ)	https://fiji.sc/	N/A
MATLAB scripts	This paper	Available upon request