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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Clin Cancer Res. 2018 Jul 18;24(21):5458–5470. doi: 10.1158/1078-0432.CCR-18-0549

Fig. 1. MCL1 Depletion Sensitizes BH3 Mimetics to Induce Apoptosis in Prostate Cancer Cells in vitro.

Fig. 1.

(A) LNCaP or PC3 cells transfected with MCL1 siRNA or non-target control siRNA were treated with ABT-737 (0 – 5 μM) for 4 hours. Total cell lysates were immunoblotted for indicated proteins. Apoptosis induction was detected with cleaved caspase 3 (CC3) signal. SE, short exposure; LE, long exposure. (B) LNCaP cells stably expressing 5 distinct MCL1 lentiviral shRNAs or a non-target shRNA were treated with ABT-737 (500 nM) for 4 hours, followed by immunoblotting. (C) LNCaP cells stably expressing doxycycline-inducible lentiviral MCL1 shRNA were treated with ABT-737 for 4 hours with and without 48 hours of doxycycline (DOX) pre-incubation. (D) Three MCL1 deficient LNCaP subclones generated with CAS9/CRISPR and independent guide RNAs (SgMCL1) and 1 negative control clone (non-specific guide RNA, SgCtrl) were treated with ABT-263 for 4 hours. (E) LNCaP cells were transfected with MCL1 siRNA or non-target siRNA and then treated with ABT-737 (500 nM) or ABT-199 (venetoclax, BCL2 selective inhibitor), and harvested 48 hours later.