(A-D) Southern analysis of ERCs in ~90-repeat strains after shift from dextrose (YPD, t=0) to glycerol media. (A) Representative Southern blot of total ERCs. (B) Average signal of biological replicates, n=4 (*P < 0.05, **P < 0.01, ***P < 0.001). (C) Representative Southern blot of URA3-marked ERCs. (D) Average signal of biological replicates, n=4, performed in duplicate (*P < 0.05, **P < 0.01, ***P < 0.001).