Figure 3.
miR-182-5p functions as a tumor suppressor gene both in vitro and in vivo. A Flow cytometric analysis of Annexin-V-FITC -7AAD stained Caki-1 and ACHN cells transfected with miR-CON/ miR-182-5p. B Cell cycle analysis showing G2/M arrest in Caki-1 and ACHN cells. C Tumor volume following intra-tumoral injection of control or miR-182-5p mimic into established tumors. Data represents mean of each group and error bars are SEM. Day 0 is start of injection and tumor growth was followed for 28 days and each mouse received total of 9 injections. Representative pictures of mice injected with either control or miR-182-5p mimic (mean ± SE,p-value calculated by student’s t-test).