Seizure burden analysis by sex. (A - B) EEG seizure burden. PB was efficacious as an anti-seizure agent in both sexes. (C - D) Corresponding seizure suppression (%) followed similar trends for PB suppression, PB rebound and BTN. (D1) For +PB+BTN treatment group, males and females showed differences in BTN aggravation. EEG sample size: n=13 males, n=13 females. Within groups: *P < 0.05, **P < 0.01, ***P ≤ 0.001, two-way ANOVA with Bonferroni post-hoc correlations. Between groups: #P < 0.05, ##P < 0.01, ###P ≤ 0.001, one-way ANOVA with Bonferroni post-hoc correlations.