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. 2018 Oct 29;16:110–114. doi: 10.1016/j.bbrep.2018.10.009

Fig. 2.

Fig. 2

Evaluation of BM neutrophil aging in Ctrl and SCD mice treated with H2O or Abx. (A&B) Single-housed 4-month-old male mice were treated with H2O or Abx for 2 weeks. BMs were stained for flow cytometry analysis. (A) Percentage of aged neutrophils vs. total neutrophils in BMs. n = 3 mice/group. (B) Representative flow cytometry image of BM aged neutrophils of each group. (C) BM neutrophils from genotype-housed 5-month-old Ctrl and SCD female mice were purified and cultured for 2 h. Cellular aging was measured using the Cellular Senescence Assay Kit, in which the SA-βGal activity was normalized to total protein concentration. n = 3 mice/group. * p < 0.05, compared with Ctrl-H2O group; #p < 0.05 compared with corresponding H2O group; two-way ANOVA (A); T-test (C).