Table 3.
qRT-PCR primers used for validation of transcriptome analysis
| Gene ID Gene symbol |
Sequence (5′ → 3′) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|
| TREES_T100020781 (CCL24) |
F: CTGGTGGTGAAGATGACCCC | 149 | 54.0 |
| R: CACTACCTTGCCCTCACAGG | |||
| TREES_T100019229 (JCHAIN) |
F: CAGGATAGCAGGAATCCGGG | 129 | 60.4 |
| R: AAGACAGTGCCACAGAGACC | |||
| TREES_T100008796 (C1Q) |
F: TTGTAGTCGTTGACGGCCAG | 147 | 62.3 |
| R: CAACCTCTGCGTGAACTTGC | |||
| TREES_T100012017 (SERHL2) |
F: CTCTCTGCAACAGGAGTCGC | 142 | 54.0 |
| R: TTCCCTGCTCCTTCCGACTG | |||
| TREES_T100016538 (HLA-DP) |
F: CTCTCAGCAACACCGTCAGT | 129 | 54.7 |
| R: TCCAAAGAAGTCCGTGGAGC | |||
| TREES_T100007200 (untitled) |
F: CAAGCTTGGCCGGTATGAAG | 197 | 54.7 |
| R: CACGGACTGTAGCTTTGGGTTC | |||
| TREES_T100021224 (untitled) |
F: GGGAGATCAAATGTAATGATGCCC | 150 | 62.3 |
| R: TAACACCATCCACCTTCCACG | |||
| TREES_T100001931 (FN1) |
F: TGCCTATCAGCAGGGTCTTTG | 143 | 60.4 |
| R: CGGTGTCCAAGTCACCATGA | |||
| GAPDH | F: GCTGGTGCTGAGTATGTTG | 129 | 57.0 |
| R: AGTCTTCTGGGTGGCAGTGATG |