Fig. 4.
Effect of APC extract on the expression of (a) microphthalmia-associated transcription factor (Mitf) and (b) tyrosinase (Tyr) genes in B16 cells. Cells were seeded onto 100-mm dish at a density of 3 × 106 cells per dish. After overnight incubation, the cells were treated without or with APC extract (50 µg/ml) for 12 or 24 h. Real-time PCR was then used to determine the expression level of Mitf and Tyr genes. Data are expressed as the mean ± SD of three independent experiments. Statistical significance *P < 0.05 and **P < 0.01 were defined as control versus sample