Fig. 3.

Levels of H3K9me3 and H3K9me2 in blastocysts. a Co-immunostaining of blastocysts with anti-H3K9me3 and anti-H3K9me2 antibodies. Nuclei were labeled with DAPI. Scale bar = 100 μm. b Quantification analysis of immunofluorescence intensities. Data are presented as mean ± SEM. A one-way ANOVA analysis was used to determine the differences between groups. Different superscripts above columns represent significant differences between groups (P < 0.05). Numbers within columns indicate the numbers of nuclei analyzed. IVF, in vitro fertilization embryos, n = 12; C-NT, cloned embryos from cells without KDM4D treatment, n = 12; T-NT, cloned embryos from KDM4D-treated cells, n = 9. The detection was replicated 3 times