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Quantitative RT-PCR (qPCR) analysis for the expression of developmental genes in blastocysts. Data are presented as mean ± SEM. A one-way ANOVA analysis was used to determine the differences between groups. Different superscripts above columns represent significant differences between groups (P < 0.05). IVF, in vitro fertilization embryos; C-NT, cloned embryos from cells without KDM4D treatment; T-NT, cloned embryos from KDM4D treated cells
