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. 2018 Jul 27;70(5):1435–1445. doi: 10.1007/s10616-018-0238-0

Fig. 3.

Fig. 3

Coculture with primary adipocytes accelerates accumulation of TAG in stromal vascular fraction (SVF) derived preadipocytes and increases adipogenic efficiency compared to those induced with standard protocols. SVF derived preadipocytes from aortic (aPVAT), mesenteric (mPVAT), perigonadal (GON) depots were stimulated to differentiate by coculture with primary adipocytes (SPA7) or standard pharmacological induction for 7 (SP7) and 14 (SP14) days. A Induction efficiency measured as % of cells with a lipid droplet. Values are percentage of differentiated cells (cells with lipid droplets/total number of cells) ± SEM. Significant differences are indicated by letters a, b, c, d (P < 0.05, n = 3–5). B Differentiated adipocytes were stained with NucBlue™ and HCS LipidTox™ to identify nuclei and TAG, respectively. Values are mean TAG fluorescence per adipocyte ± SEM. Significant differences are indicated by letters a, b, c (P < 0.05, n = 4). C Representative (n = 3–5) high-resolution images of aPVAT, mPVAT, GON as preadipocytes and after differentiation by SPA7, SP7, and SP14. Cells’ nuclei were stained with NucBlue™ (blue) and triacylglycerides were stained with HCS LipidTOX™ (red). Scale bars represent 50 microns. (Color figure online)