Fig. 4.

Coculture with primary adipocytes enhances the expression of adipogenic genes in stromal vascular fraction (SVF) derived preadipocytes compared to those induced with standard protocols. SVF-derived preadipocytes from aortic (aPVAT), mesenteric (mPVAT), perigonadal (GON) depots were stimulated to differentiate by coculture with primary adipocytes for 7 days (SPA7) or standard pharmacological induction for 14 days (SP14). A The effect of culture type on gene expression of adipogenic markers on cells from all sites (aPVAT, mPVAT, and GON). SVF before induction and gonadal white adipose tissue (WAT) were used as negative and positive controls. B Effect of anatomical site on the gene expression of adipogenic markers in differentiated adipocytes from SPA7 and SP14. C Effect of anatomical site on the expression of brown adipocyte markers in differentiated adipocytes from SPA7 and SP14 and adipose tissues collected from aPVAT, mPVAT and GON. Gene expression was evaluated by RT qPCR and normalized to Actb, B2m, and Rps29. Data are mean ± SEM (n = 6). ND = non detected. Significant differences are indicated by * and letters a, b, c, and d (P < 0.05), and *** (P < 0.001)