Fig. 5.

Coculture with primary adipocytes enhances the lipolytic response to isoproterenol and adiponectin secretion in stromal vascular fraction (SVF) derived adipocytes compared to those induced with standard protocol. Preadipocytes from aortic (aPVAT) and mesenteric (mPVAT) perivascular adipose tissue and perigonadal (GON) adipose depots were differentiated by coculture with primary adipocytes for 7 days (SPA7) or standard protocol for 7 (SP7) and 14 days (SP14). A Lipolysis was stimulated with isoproterenol and released glycerol was quantified. Values are mean fold change over basal glycerol release ± SEM and adjusted to cell number (n = 5). B Adiponecitn release was quantified in the medium at the end of the induction period using an ELISA kit. Values are ng of adiponectin per mL per ng of protein. Significant differences are indicated by letters a, b, and c (P < 0.05)