Fig. 8.

Blocking mTOR affects cellular senescence. BMSCs were induced with 10 g/l d-gal, 200 μM ascorbic acid or 100 nM Rapamycin. a BMSCs were stained with ROS fluorescence. Scale bar = 25 μm. b The protein expressions of p16 were measured by Western blotting (*P < 0.05). c The protein expression of p-mTOR was measured by Western blotting (*P < 0.05). d The senescent cells were examined by SA-β-gal staining (*P < 0.05). Scale bar = 25 μm. Results represent at least three independent experiments