Figure 1.

RNA-Seq Analysis of Naive and MHV68-Expanded ORF8 TCR TN CD8⁺ T Cells

(A) Schematic of the experiment. 50 × 10³ Rag1^−/− K^b-ORF8 TCR TN CD8⁺ T cells were adoptively transferred in CD45.1 congenic C57BL/6 mice, and recipients were infected with 5 × 10⁵ plaque-forming unit (PFU) of MHV68 i.p. On 6 dpi, spleens were isolated and single-cell suspensions were analyzed flow cytometrically using a panel of indicated cell surface markers.

(B) Representative FACS plots showing the phenotypic markers expressed by CD45.2-positive cell (Rag1^−/− K^b-ORF8 TCR TN CD8⁺ T cells) activated and expanded in response to viral infection.

(C) Sorted CD45.2⁺ CD44^hi cells that were expanded due to MHV68 infection and their naive counterparts were used for RNA sequencing. Scatterplot shows the differential expression of transcripts in naive and 6 dpi activated TN CD8⁺ T cells.

(D and E) (D) Bar diagram shows the extent by which the fraction of genes are differentially expressed. (E) Gene ontology panther pathway analysis of differentially expressed transcripts in ORF8 TCR TN CD8⁺ T cells for the biological processes represented.