Figure 1.

Identification of Histone Demethylases Engaged in Regulation of p53 Function

(A) Schematic showing the p21 luciferase reporter bearing two p53 binding sites that are subject to modulation by histone demethylases.

(B) Screening results showing the relative luciferase activity that is driven by p53 after siRNA knockdown of the indicated genes.

(C) RT-PCR for CDKN1A after 72-hr knockdown of KDM5A. Data are mean ± SEM.

(D) Genetic amplification and deletion of histone demethylases across 10,844 cancers. Circled red dots indicate that gene amplification is at its peak. Cutoff q value is 0.25.

(E) Contingency table for the analysis of the distribution frequency of genetic amplification/loss of KDM5A from TCGA PAN-CAN UCSC datasets.

(F) Amplification of KDM5A or MDM2 and TP53 mutations from TCGA PAN-CAN UCSC data. Chi-square test for statistical analyses, p < 0.001.

(G) The genetic alteration of TP53, MDM2, KDM5A, and CDKN2A from five cancer cohort data.

(H) The association of genetic alteration of TP53, MDM2, KDM5A, and CDKN2A from five cancer cohort datasets.