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. 2018 Oct 10;11:73–82. doi: 10.1016/j.omtm.2018.10.002

Figure 5.

Figure 5

miR-96 and miR-21 Have an Additive Effect on the Inhibition of Smad7 In Vitro

(A) RT-PCR analysis of smad7 expression in mouse primary HSCs transfected with mimics of miR-21, miR-96, the combination of miR-21 and miR-96, or controls. (B) Western blot analysis of SMAD7 in the HSC-T6 cell line transfected with inhibitors of miR-21, miR-96, the combination of miR-21 and miR-96, or controls. (C) Western blot analysis of SMAD7 expression in liver samples from uninfected and infected mice treated with the rAAV8-anti-miR-96-TuD vector, the rAAV8-anti-miR-21-TuD vector, the combination of both vectors, or control vectors. (D) RT-PCR analysis of smad7 expression in mouse primary HSCs isolated from uninfected and infected mice treated with the rAAV8-anti-miR-96-TuD vector, the rAAV8-anti-miR-21-TuD vector, the combination of both vectors, or the control vector. Data are represented as mean ± SD. *p < 0.05; **p < 0.01, compared between indicated groups.