Fig. 6.

DAPI and EtBr-acridine orange staining of AGS cells. AGS cells were grown and treated as described and stained with (a) DAPI (top row panels) and (b) EtBr-acridine orange cocktail (bottom row panels), which revealed the nuclear morphology as well as the apoptotic state depending on the membrane integrity. Inset pictures in the panel-a show the 60X magnified form. (c) It was seen that viability of cells did not decrease significantly on 5 min. exposure for both the concentrations. When the exposure time was further increased for more 15 min., the viability decreased by 50% in case of 2.5% ethanol treated cells whereas viability decreased to ∼25% in 5% ethanol treated cells. We chose the damaging concentration of ethanol as 2.5% and time as 30 min. Beyond that, the cells were excessively damaged.