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. 2018 Nov 2;8:16245. doi: 10.1038/s41598-018-34631-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scattergram analysis of peripheral blood from X. laevis. (A) Scattergram indicates forward-scattered light (FSC) and side-scattered light (SSC). (B) Scattergram indicates the F530 and F695 fluorescence intensity. (C) X. laevis blood cell population LP1, LP2, and debris fractions developed based on the cellular red fluorescence intensity (F695/FSC) and cellular red fluorescence intensity (F530/FSC). (D) LP3 and LP4 were determined based on the F695 fluorescence intensity. (E) LP2, LP3, and LP4 were identified based on FSC and SSC. LP2 and LP3 partially overlapped, and LP4 completely overlapped with LP2 and LP3. (F) LP2, LP3 and LP4 were determined based on the F530 and F695 fluorescence intensity. LP2: Erythrocytes (grey); LP3: Granulocytes (Red); LP4: Lymphocytes and Thrombocytes (Blue).