Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 2;9:4600. doi: 10.1038/s41467-018-06833-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Isolation of the causative lesion and expression pattern of Myh10. a Integrative genomics viewer (IGV) snapshot of the c.T1373G mutation in Myh10 (chr 11:68,765,165). b Normalized melting curves showing the Myh10 mutation by high-resolution melting analysis (HRMA). c Chromatogram of two different genotypes of Myh10 by Sanger sequencing. d Schematic diagram of MYH10 protein domains and the relative position of the L458 residue, which is conserved from worms to humans. e In situ hybridization for Myh10 expression in E13.5 whole-mount and on cryosectioned lungs; e epithelium. f Double staining for Tbx4 mRNA (red) and MYH10 protein (green) on E16.5 lung sections; e epithelium. g Immunostaining for MYH10, E-cadherin (marking epithelial cells), α-SMA (marking myofibroblasts and smooth muscle cells), PDGFR-β (marking myofibroblasts and smooth muscle cells), and NG2 (marking pericyte-like cells) on E16.5 and E18.5 lung sections; b blood vessel. Scale bars: 200 μm (e (left)), 50 μm (e (right), f (left)), 20 μm (f (right), g (left)), 10 μm (g (right))