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. 2018 Nov 2;9:4600. doi: 10.1038/s41467-018-06833-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Myh10 deficiency leads to disrupted ECM remodeling. a Immunostaining and quantification for Fibronectin, type I collagen (COL I), and Tropoelastin on Myh10^+/+ (n = 10) and Myh10^-/- (n = 10) E18.5 lung sections. b Representative TEM images comparing elastic fibers in Myh10^+/+ (n = 3) and Myh10^-/- (n = 3) E18.5 lungs. Asterisks and arrowheads point to bundles and fragmented bundles of elastic fibers, respectively; c collagenous fibrils. c Representative western blot (from three individual sets of lung lysates) of Myh10^+/+, Myh10^+/-, and Myh10^-/- lung lysates at E18.5. Values represent the densitometric ratio of Myh10^+/- and Myh10^-/- to Myh10^+/+ after normalization to GAPDH. d Immunostaining (from five individual sets of cultured cells) for Fibronectin in DMSO, GM6001, or MMP2 inhibitor I-treated Myh10^+/+ and Myh10^-/- embryonic fibroblasts. e Immunostaining and quantification for Fibronectin, COL I, and Tropoelastin on Myh10^-/- (n = 5) and GM6001-injected Myh10^-/- (n = 5) E18.5 lung sections. f Expression level of Mmp2 in EGFP siRNA and Mmp2 siRNA-treated Myh10^+/+ embryonic fibroblasts (from three replicates and individual sets of cultured cells) by RT-qPCR. g Immunostaining and quantification for Fibronectin in EGFP siRNA and Mmp2 siRNA-treated Myh10^+/+ and Myh10^-/- embryonic fibroblasts. Error bars are means ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed Student’s t-test. Scale bars: 30 μm (a, e), 20 μm (d, g), 500 nm (b)