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. 2018 Nov 2;9:4600. doi: 10.1038/s41467-018-06833-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Mesenchyme-specific Myh10 deletion disrupts lung development. a Diagram indicating time points of tamoxifen injections and tissue collection. Immunostaining for E-cadherin in Gli1-Cre^ERT2;ROSA26^tdTomato P0 lungs. b Gross morphology of wild-type (n = 10) and Gli1-Myh10^cKO (n = 8) P0 mice. Arrowheads point to inflated (left) and non-inflated lungs (right). c Floating assay for wild-type (n = 10) and Gli1-Myh10^cKO (n = 8) P0 lungs. d Representative pictures of distal lung from wild-type (n = 10) and Gli1-Myh10^cKO (n = 8) P0 mice. e H&E staining and morphometric analysis of alveolar space and septal thickness in wild-type (n = 10) and Gli1-Myh10^cKO (n = 8) lungs at P0. f Immunostaining for NKX2-1, PCNA, and SFTPC in wild-type (n = 10) and Gli1-Myh10^cKO (n = 8) P0 lungs. g Quantification of cell proliferation and differentiation in wild-type and Gli1-Myh10^cKO P0 lungs. h Immunostaining for fibronectin, tropoelastin, and tdTomato in wild-type (n = 10) and Gli1-Myh10^cKO (n = 8) P0 lungs. Error bars are means ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed Student’s t-test. Scale bars: 500 μm (d), 50 μm (e), 30 μm (a, f, h (left, middle)), 15 μm (h (right))