Fig. 5.

Y288C promotes cell proliferation through Akt, ERK1/2, and STAT3. MCF10A cells stably expressing Y288C was transfected with siRNA specifically target, Akt1, MAPK1/3, STAT3, or STAT5, respectively. a The knockdown efficiency was verified by western blot 48 h after transfection. 24 h after transfection, cells were trypsinized, counted and plated for b cell viability assay (Student’s t-test) and c 3D growth in growth factor-reduced Matrigel for 4 days. The relative cell viability of three independent replicates was assessed with Prestoblue 4 days after transfection. Bar, 100 μm. The area of 50 spheroids are presented as mean ± SD (Dunnett’s multiple comparisons test). ***p < 0.001