Fig. 1. TBM inhibits cervical cancer cells proliferation.

a Hela and SiHa cells were treated with indicated concentrations of TBM for 24 h. Cell viability was measured by the MTT assay. b TBM disrupted cellular membrane integrity as measured by LDH release in the medium. Cells were treated as in (a). c–d Cell proliferation of HeLa and SiHa cells were measured by EdU labeling. Cells were treated as in (a). Scale bars: 100μm. e–g Nude mice bearing HeLa xenograft tumor were treated with 100 μL saline solution (control, n = 5) or 3 mg/kg TBM (n = 5) daily for 16 days. e Tumor tissues were taken and imaged after animals sacrificed. Scale bars: 1 cm. f Tumor volumes were monitored every other day and calculated by the length and width. g Tumors were weighed immediately once mice were killed. h–i Tumor tissues were sectioned and subjected to immunohistochemistry for evaluating expression of Ki67. Scale bars: 100 μm. *p < 0.05; **p < 0.01; ***p < 0.001