Fig. 2. TBM induces apoptosis in cervical cancer cells both in vitro and in vivo.

a–b HeLa and SiHa cells were treated with TBM (15 μM) either alone or combination with specific cell death inhibitors, ferrostatin-1 (5 μM), necrostatin-1 (5 μM), Z-VAD-FMK (10 μM) and CQ (10 μM) for 24 h, and the percentage of cell death was determined by MTT assay. c–d HeLa and SiHa cells were treated with indicated concentrations of TBM for 24 h, and apoptosis index was determined by TUNEL assay. Scale bars: 100μm. e HeLa and SiHa cells were treated with indicated concentrations of TBM for 24 h, and then apoptosis was measured with FACS assay. f HeLa and SiHa cells were treated with indicated concentrations of TBM for 24 h, and the expression of cleaved CASP3 and PARP1 was measured with immunoblot. g Tumor samples were collected from the mouse xenograft mode, and the expression of cleaved CASP3 and PARP1 was measured with immunoblot. *p < 0.05; ***p < 0.001