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. 2018 Nov 2;9(11):1117. doi: 10.1038/s41419-018-1151-3

Fig. 7. Accumulation of impaired autophagolysosomes is contributed to TBM-induced cell death.

Fig. 7

a Cells were transfected with siScramble, siATG5 or siBeclin1 for 48 h. The expression of Atg5 and Beclin1 was measured by immunoblot. The ratio of Atg5/β-actin and Beclin1/β-actin was determined with ImageJ software. b–c Cells were transfected with siScramble, siATG5 or siBeclin1 for 48 h, and then treated with 15 μM TBM for another 24 h. b Cell viability was detected by MTT assay. c Proliferation rate was detected by EdU labeling. d Cells were treated with 15 μM TBM in the absence or presence of 3-MA or WTM. Cell viability was determined by MTT assay. e Cells were treated with 15 μM TBM in the absence or presence of bafilomycin A1 (Baf). Cell viability was determined by MTT assay. *p < 0.05; ***p < 0.001