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. 2018 Nov 2;8:16238. doi: 10.1038/s41598-018-33233-0

Figure 3.

Figure 3

Early induction of HO-1 in Mdr2−/− mice may interfere with hepatocellular proliferation. (A) Hepatic mRNA expression levels of Cyclin D1 determined by quantitative real time RT-PCR in the livers of Mdr2−/− mice treated with either PBS or CoPP as described in Suppl. Fig. 1A, normalized to 5-week-old WT  mice (n ≥ 5). (B) Hepatic protein expression level and phosphorylation state of p38 determined by western blot (WB) analysis of 36-week-old Mdr2−/− mice treated as described in Suppl. Fig. 1A compared to WT mice of the same age (n ≥ 3). (C) Quantification of the WB described in (B). (D) Hepatic protein expression level and phosphorylation state of p38 determined by western blot (WB) analysis of 65-week-old Mdr2−/− mice treated as described in Suppl. Fig. 1A compared to WT mice of the same age (n ≥ 3). (E) Quantification of the WBs described in (D). The samples for the p38 and p-p38 WBs were derived from the same experiment and gels/blots were processed in parallel. Images of the full length blots are presented in Supplementary Fig. 4. Data are expressed as means ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.