Table 3.
Antibodies for immunohistochemistry.
| Target | Host | Dilution | Distributed by |
|---|---|---|---|
| F4/80 | rat | 1:200 | #MCA497R, Bio Rad, Hercules, CA |
| HNF4-alpha | goat | 1:100 | #sc-6556, Santa Cruz, Dallas, TX |
| γH2A.X | rabbit | 1:200 | #2577, Cell Signaling, Danvers, MA |
| anti-Rabbit IgG (H + L) Alexa Fluor 488 | goat | 1:200 | #A-11008, Life Technologies Carlsbad, CA |
| anti-Rat IgG (H + L) Alexa Fluor 594 | donkey | 1:200 | #A-21209, Life Technologies, Carlsbad, CA |
| anti-Phospho-Histone H2A.x | mouse | 1:200 | #05-636, Millipore, Burlington, MA |
| anti-mouse ALEXA fluor 594 | goat | 1:600 | #A-11005 Thermo Fisher, Waltham, MA |
HNF4: hepatocyte nuclear factor 4; γH2AX: phosphorylated histone H2AX; IgG: immunoglobulin.