Fig. 5.

Human brain CD8⁺ T cells show a T-bet-intermediate/eomes-low phenotype. a Dot plot showing the gating strategy T-bet and eomes in a paired blood and brain sample. T-bet and eomes co-expression (lo/lo > int/lo > lo/hi > int/hi > hi/hi > hi/lo) correlates in virus-specific CD8⁺ T-cell with differentiation from central memory to terminally differentiated effector cells, respectively¹⁵. b Comparison between blood and brain CD8⁺ T_RM cells, and stratification based on CD69 and CD103 co-expression of brain eomes low (c) and all T-bet/eomes subsets (d). e Dot plot of T-bet and Hobit co-expression by CD8⁺ T cells from brain and blood of a donor. f Quantification of Hobit-positive cells is shown. Bars show median values. p-values show Mann–Whitney U test (b, f) and Friedman test with Wilcoxon signed ranks as post hoc test (c, d) (*p < 0.05, **p < 0.01); no brackets indicate no significant difference