Fig. 3.

Epithelial Stat3 deletion changes the lung tumor microenvironment in a sex-dependent manner. Total inflammatory cell and lineage-specific leukocyte numbers from bronchoalveolar lavage fluid (BALF) of female (n = 7) (a) and male (n = 5–7) (c) CC-LR and LR/Stat3^Δ/Δ mice at the age of 14 weeks. Relative expression of Arg1, Ido, Cxcl1, Ccl2, Il6, Tgfb, Il17, Tnfa, Ifng and Gzmb mRNA in whole lungs of female (b) and male (d) CC-LR and LR/Stat3^Δ/Δ mice at the age of 14 weeks, normalized by CD45 expression (data represent means ± SEM; ^***P < 0.001; ^**P < 0.005; ^*P < 0.05 using two-tailed t-test, experimental replicate # 2-3). WBC, white blood cell