Fig. 6.

FGF21 stimulates Oxt expression through AKT. a Hypothalamic Oxt mRNA expression levels after IP injection of mFGF21 (1 mg/kg) or water (vehicle) (n = 4 per group). b, c The relative expression of Oxt mRNA in hypothalamic N41 cells after mFGF21 treatment: time-course data (b) and dose-response data (c) (n = 3 per group). d Luciferase activity of Oxt promoter of −5000 bp in N41 cells was measured in response to 100 nM mFGF21 and vehicle (water) (n = 3 per group). e Western blot analyses of N41 lysates after 100 nM mFGF21 treatment for 0, 5, 10 or 15 min (n = 3 per time point). f Effect of inhibiting ERK signalling or AKT signalling on FGF21-induced Oxt expression. N41 cells underwent 30-min pre-treatment with or without the ERK inhibitor (10 μM U0126) or the PI3K inhibitor (10 μM wortmannin); subsequently, cells were incubated for 8 h in the absence or presence of 100 nM mFGF21 (n = 4 per group). Data are shown as box and whisker plots (centre line, median; box limits, upper and lower quartiles; whiskers, the minimum and maximum value of a data set). *p < 0.05. Data in a, b, c, d, f were replicated more than three times, and data in e were replicated twice in the laboratory