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. 2018 Nov 2;8:16277. doi: 10.1038/s41598-018-34460-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pharmacological manipulation of DA reuptake. Nomifensine (800 µM), a DA reuptake inhibitor, was added to the brain perfusate 10 min prior to stimulation with electric shock (10 V, 100 ms). (A) Representative traces recorded before treatment (Control, Ctrl), after 10 min exposure to nomifensine (Nom) and following a 10 min saline wash (Wash). (B) Maximum DA release (mean + SEM). Nomifensine did not alter the amplitude of DA release. (C) Clearance rate (mean + SEM). Nomifensine (800 µM) slows DA reuptake. Data presented in this figure represent 55 measurements from 5 bees.