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. 2018 Nov 2;8:16283. doi: 10.1038/s41598-018-34536-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The spatio-temporal coordination of membrane Gag assembling VLPs. (a) Box plot of the maximum effective trapping energy observed in fully assembling VLPs. (b) Normalised distributions (bars) and their log-normal fits (plain lines) of the diameters at full width at half maximum (FWHM) of the maximal intensity attractive potentials for WM (blue), WT Gag (black) and NL4.3ΔPolΔEnv Gag (red). Dashed line represents the log-normal fit of the assembling clusters distribution observed in fixed cells and depicted in Fig. 2. (c) Normalised distributions of the difference between the time to reach the maximum localisation density increase (LDI) and the time to reach the maximal energy trapping for WM (blue), WT Gag (black) and NL4.3ΔPolΔEnv Gag (red). Distributions were significantly different according to the Kolmogorov-Smirnov test (see Table S4 for values).